Undergrad Thesis – Crown Ecozyme™

Crown Ecozyme™ | Undergrad Thesis | Formulation | TDS | MSDS

Undergraduate Thesis Proposal

Title: Effect of Crown Ecozyme™ on Soil Enzymatic Activity and Growth Performance of High-Value Crops

1. Introduction / Background

Soil enzymatic activity plays a pivotal role in nutrient cycling, organic matter decomposition, and overall soil fertility. Enzymes such as cellulases, proteases, amylases, and pectinases catalyze critical biochemical processes that influence plant nutrient availability.

Crown Ecozyme™, developed by Crown Organic Fertilizers Inc., is a bio-enzymatic soil and plant booster designed to enhance soil enzymatic activity, stimulate microbial populations, and improve plant growth. Its formulation combines bioactive enzymes, beneficial microbes, and organic nutrients to create a synergistic effect that revitalizes soil and promotes vigorous crop development.

High-value crops, such as Agarwood, perfumery plants, fruit trees, and specialty vegetables, benefit from enhanced enzymatic soil activity, which supports stronger root systems, improved nutrient uptake, and better overall plant performance. Systematic evaluation is required to quantify the effects of Ecozyme™ under controlled conditions.

2. Research Problem / Statement

There is limited empirical data on the efficacy of Crown Ecozyme™ in enhancing soil enzymatic activity and promoting plant growth. This study aims to answer:

  1. How does Crown Ecozyme™ affect soil enzymatic activity and microbial populations?
  2. What impact does Ecozyme™ have on plant growth parameters such as height, leaf area, root development, and biomass?
  3. Does Ecozyme™ improve crop yield and overall plant health compared to conventional fertilization methods?

3. Objectives

General Objective:

To evaluate the effects of Crown Ecozyme™ on soil enzymatic activity, microbial populations, and growth performance of selected high-value crops.

Specific Objectives:

  1. To measure changes in soil enzymatic activity (cellulase, protease, amylase, pectinase) following Ecozyme™ application.
  2. To assess soil microbial population dynamics after Ecozyme™ treatment.
  3. To evaluate plant growth parameters, including height, stem diameter, leaf area, root length, and biomass.
  4. To compare crop yield and quality between Ecozyme™-treated plants and control groups using conventional fertilizers.

4. Hypothesis

Null Hypothesis (H₀): Crown Ecozyme™ does not significantly affect soil enzymatic activity, microbial populations, or plant growth compared to conventional fertilization.
Alternative Hypothesis (H₁): Crown Ecozyme™ significantly enhances soil enzymatic activity, microbial populations, and plant growth compared to conventional fertilization.

5. Scope and Delimitation

  • Crop Selection: High-value crops such as Agarwood seedlings, perfumery plants, fruit trees, or specialty vegetables.
  • Study Duration: 3–6 months for vegetative growth; yield assessment where applicable.
  • Parameters: Soil enzymatic activity, microbial populations, plant growth metrics, and crop yield.
  • Limitations: Environmental factors (temperature, rainfall, humidity) may influence outcomes; study may be limited to nursery or small field plots.

6. Significance of the Study

  • For Farmers: Provides insights on sustainable enzyme-based soil enhancement to improve plant growth and soil health.
  • For Academia: Contributes to understanding enzyme-microbe-plant interactions in agroecosystems.
  • For Crown Organic Fertilizers Inc.: Provides empirical data to support Ecozyme™ adoption and credibility as a bio-enzymatic product.

7. Methodology

7.1 Experimental Design

  • Design Type: Completely Randomized Design (CRD) or Randomized Complete Block Design (RCBD)
  • Treatments:
    1. Control (no fertilizer)
    2. Conventional chemical fertilizer
    3. Crown Ecozyme™ at recommended dosage
  • Replications: Minimum of 3 per treatment

7.2 Data Collection

  • Soil Analysis: Enzymatic activities (cellulase, protease, amylase, pectinase), microbial population counts, and nutrient analysis (N, P, K, organic carbon)
  • Plant Growth Parameters: Height, stem diameter, leaf area, root length, biomass
  • Yield Parameters: Biomass or product yield (where applicable)

7.3 Data Analysis

  • Statistical analysis using ANOVA to determine significant differences between treatments
  • Post-hoc tests (Tukey’s HSD) for multiple comparisons
  • Significance level: p < 0.05

8. Expected Outcomes

  • Crown Ecozyme™ is expected to enhance soil enzymatic activity and microbial populations, promoting nutrient cycling and root growth.
  • Treated plants should exhibit improved vegetative growth, root development, and stress resilience.
  • Crop yield and quality are anticipated to improve compared to control and conventional fertilizer treatments.

9. References (Sample)

  1. Burns, R. G., et al. (2013). Soil enzymes in the context of global change. Soil Biology & Biochemistry, 58, 1–14.
  2. Maheshwari, D. K., & Saraf, M. (2017). Biofertilizers and sustainable agriculture. Journal of Plant Nutrition and Soil Science, 180(2), 219–232.
  3. Crown Organic Fertilizers Inc. (2025). Crown Ecozyme™ Technical Data Sheet.